Author Topic: videos and tips for crossing technique  (Read 107 times)

Steph S

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videos and tips for crossing technique
« on: 2021-07-10, 05:21:34 PM »
Just thought I would start a thread here for anyone to post their favorite videos or tips on the hand crossing technique, for anyone who is starting out and making their first crosses.
There are dozens of videos that show the process of choosing a flower to emasculate, and quite a few different ways of doing it.   This video is I think my personal favorite, closest to the way I do it myself, except that I use my fingernails instead of tweezers to clip the sepals and petals, then remove a strip of the anther cone first so the rest of it can be pulled away from the exposed stigma.
https://www.youtube.com/watch?v=rPOXEDS9yIo

I've tried using tweezers but it seemed like I was inflicting more trauma on the bud than when I just use my handy sharp nails.  There are some different and very cool techniques out there in the videos, more dexterous than I will likely try to be (but I can admire it!).

Something I learned from experienced breeders is that the stigma is usually not fully mature on the day you emasculate the flower.  So although I apply pollen immediately, I also repeat that for two more days.  When the stigma is mature and receptive it forms more of a knob on the end and becomes sticky, and you can see that it clears a line as you drag it through the pollen.  Then you know you got it pollinated when it was ready.

Another tip which iirc came from Tom Wagner, is to collect pollen from several donor flowers.  This way you have pollen at different stages of maturity in the mix, and increases the chance of getting the right stuff to your mature stigma.  Personally I use one container for each variety pollen, and continue to collect fresh into that for my three day process, from multiple donor flowers on the plant.    I have some small containers with shallow lids, so I turn them upsidedown, use the lid to collect and dispense pollen (which is shallow enough to dip your stigma into), and use the container bottom as the 'lid' to keep it closed in the cool basement where it waits for next day action.

Please share your favorite resources and tips, and tell us your personal favorite techniques.


William S.

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Re: videos and tips for crossing technique
« Reply #1 on: 2021-07-10, 06:42:39 PM »
When I did my two mission mtn sunrise x big hill earlier I used fresh big hill pollen each day for three days and I emasculated younger flowers. Then I clipped all competing flowers with my fingernails. I don't think I used tools because of relatively sturdy flowers. I may have, just can't recall. Still waiting to pick those two fruits but I am pretty sure I nailed it.

I have needed my botanical forceps for slender stigma emasculations. If I were doing a lot I would use tools. Though I have been using botanical forceps almost my entire adult life to dissect plants.

I find that my flowers dry up when emasculated outside. Especially now when it is maximally hot, dry, and sometimes windy.

So what I do instead is utilize exserted mothers but when I really want a cross that way I transfer pollen still manually. It works great if the mother is potato leaved and father regular leaf as F1's will be regular leaf. Which is why I've selected Mission Mountain Sunrise for potato leaf like the unknown Lofthouse landrace that was it's own cross mother. It's also why I've crossed MMS to BH because BH has better exsertion. MMS is inserted to modestly exserted. I'm setting myself (and others hopefully) up for easier future crosses.

Other traits that help ID crosses in F1: red is dominant over yellow. Non-rugose over rugose. Stripes on fruit are recessive. 

Sometimes you can see the pollen especially through a hand lens or macro lens on a camera before and after absent then adhered.

I think doing a few crosses than growing lots of F2s might be more important to achieving trait combination goals than lots of crosses than growing out few of each line.
« Last Edit: 2021-07-10, 11:10:08 PM by William S. »
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galina

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Re: videos and tips for crossing technique
« Reply #2 on: 2021-07-15, 06:57:28 AM »
Central England, cool, maritime (ish), cloudy, often dry, but recent weather unpredictable

Steph S

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Re: videos and tips for crossing technique
« Reply #3 on: 2021-07-15, 08:00:38 AM »
That's really a superb video Galina!  Thanks.  :)

Garrett Schantz

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Re: videos and tips for crossing technique
« Reply #4 on: 2021-07-15, 05:45:17 PM »
I like this video for tomatoes - some other things.

https://www.youtube.com/watch?v=C1Riedng9WY&t=


This one is nice for peas.

https://www.youtube.com/watch?v=zMEnXXzmV3s&t=


There are a lot of nice videos on YouTube.

Adrian

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Re: videos and tips for crossing technique
« Reply #5 on: 2021-07-16, 05:53:32 AM »
Thank you for your video! I will try to hybrided a tomato.
I have think at yellow pear x san marzano 2

Adrian

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Re: videos and tips for crossing technique
« Reply #6 on: 2021-07-21, 01:50:14 PM »
This link is in french but he has a lot of pictures extremly interested.
https://jardinierparesseux.com/2018/07/15/comment-creer-votre-propre-tomate-hybride/
« Last Edit: 2021-07-21, 02:00:12 PM by Adrian »