Hybrid of tomato more diversity

[Adrian]

Hello,
It look more and more important to hybrided the tomatos: this year is the pur exemple.All my tomatos have caught the blight this year and i have see most dangerous i have note that a few tomatos under a shelter have a few leafs with the blight.(this shelter is may old not totaly waterthight.)
The blight is not the alone problem for example: resistivity at the mosaïc, shadow, cold, hot, drought, rain
I don't demand a tomato totaly resistiant i have understand it impossible, the most resistant tomatos today could be the less resistant tomorow i want just more diversity on my patch.
For realised this experience, i will take a few cutings of tomatos or sow a few tomatos (if the plants are too sick)  that i will isolated in pot.If it possible i will put this pot at a placement without wind for avoid the autofecondation.If a fruit get fat i will know easily if my fecondation is good.

[Ocimum]

Hello Adrian,

I am not sure if I understand you well: do you want to let cross pollination happen without you helping, just by stopping wind? That would not work, as a bumble bee would also vibrate the flower and induce self pollination.
Cross pollinating tomatoes is quite easy to do by hand, there are many videos to show the process https://www.youtube.com/results?search_query=crossing+tomatoes

And I am sure quite a few people here share seeds of F2 or F3 as well, to help kickstart your selections. The outcrossing tomato project is one which is the most promising to avoid the hand pollination, as it takes time and requires to be around at the right time.

[William S.]

You might be able to get EFN to send you seeds of something like Exserted Orange or Big Hill with a somewhat higher outcrossing rate. Or the promiscuous project. Or just look for exserted stigmas I find I can get a Sungold or Sunsugar with good exsertion regularly at the local hardware store when they sell plant starts in the spring.

[Adrian]

For this moment i grow andean horned, san marzano 2 and canestrino di lucca and yellow pear.
I don't think they are very resistantvthey have may the PH1 gene.But i think a few with exsert stigmats.

[Adrian]

This flower is exserted?
Its the variety canestrino di lucca.

[William S.]

Yes exserted but very modestly so. This type of exsertion might get a rare hybrid. Emasculation of the anthers before pollen release and then pollen transfer would still be needed for better success.

[Adrian]

Thank you william.I will try to put the male pollen of horned andean!

[Adrian]

It important the model of paintbrush for take the male pollen?

[Adrian]

It normal i have not the impression to see the male pollen on the paintbrush after this stage?

[Steph S]

I've never used that paintbrush technique, Adrian.  It's fairly easy to collect pollen simply by tapping an open flower onto a smooth, fairly flat surface (for example, the lens from an old pair of sunglasses is suggested).   The pollen is then applied to the stigma by gently dragging it across the pollen coated surface.
If you want your seed to be dependably hybrid, take off the anther cone of your mother flower.  Otherwise by the time it is fully opened and exserted it may be already self pollinating. 

[Adrian]

I have tryed this morning
I have put the male anther on the stylet as the video!
I have not sucessed to remove the female anther but i have open this.
Unfortunately the plant or i have did the cross has caught the blight.
I am very choqued to see the tomatos caught the blight under a shelter!It never arrived so early.

[Adrian]

First polinisation:andean x canestrino
Second polinisation: canestrino x san marzano 2
It look not so difficult finaly.
I don't know if thr fruits will get fat but it funny!
I have sucessed to remove the anther!

[Adrian]

 the exsert stylet is a dominant gene?

[William S.]

The exserted stigma seems recessive or codominant to me. Also it is probably at least two different genes or alleles.

We see roughly four easily observable conditions.

1. Deeply inserted

2. Near the tip of the anther cone

3. Modestly exserted

4. Extremely exserted

I think plants with extreme exsertion sometimes cross with insertion and produce modest exsertion = codominance.

Sometimes modest exsertion is relatively stable and must be a different allele or gene than extreme exsertion.

Sometimes exsertion seems environmentally enfluenced hotter = greater exsertion. This might be only some alleles.

Blight resistant varieties might be useful for crossing.

[Adrian]

 Thanks you it very interessant!
If it a sucess i will try to selected  all the modestely exsert stigmat.
Must i change the flower if the anther is dry after a few hours or it sufficient for protected the pollen?