Author Topic: CRISPR for sale  (Read 197 times)

Diane Whitehead

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CRISPR for sale
« on: 2019-09-21, 10:07:45 AM »
There is a company that will sell us a gene snipper and a gene snippet to put in the gap.

I wonder how much it costs.

This is from an ad accompanying an article in EatingWell

Synthego
CRISPR Design Tool

World’s Fastest & Easiest CRISPR Gene Knockout Design Tool
Victoria, British Columbia, Canada
cool mediterranean climate  warm dry summers, mild wet winters,  70 cm rain,   sandy soil

Walt

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Re: CRISPR for sale
« Reply #1 on: 2019-09-21, 08:11:58 PM »
Interesting.  But the hard part now is locating which genes you want snipped in or out.

ImGrimmer

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Re: CRISPR for sale
« Reply #2 on: 2019-09-24, 03:07:23 AM »
I have an idea :)
using the ecf gene in Citrus would probably lead to a plant that flower within 1 year.
like done in plums
Further breeding for other breeding goals would be the same as usual but with 1 generation per year.
There are other genes e.g. in Arabidopsis causing the same. Even genes from C.wakonai or papuana that causes early maturity would be interesting. Wakonai hybrids had poor fruit quality even after some breeding generations. (personal correspondence with M.Smith Bundaberg) With CRIPSR the precocious flowering genes could be transferred.
Dreaming :)

ImGrimmer

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Re: CRISPR for sale
« Reply #3 on: 2019-09-24, 03:26:58 AM »
I don`t understand how it works for incorporating a gene but unit price for one gene knock out would be $225.

Walt

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Re: CRISPR for sale
« Reply #4 on: 2019-09-24, 09:58:47 AM »
The precocious Poncirus trifoliata Laaz found and has distributed might have the ecf gene, or a gene with similar effect.  Yet after more than a decade, it is not in any reported F2 hybrids.*  I presume if ecf was CRISPred into citrus it would also be recessive, and it would be a while before it was used.
But I'm growing several of Laaz's precocious poncirus and I'll be using it as soon as I can.  But it won't make a difference for several years.


* see http://tropicalfruitforum.com/index.php?topic=24086.0

ImGrimmer

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Re: CRISPR for sale
« Reply #5 on: 2019-09-24, 01:36:32 PM »

This precocious Poncirus is a strange plant.
For me it doesn't grow at all. So far I have lost all seedlings and grafts. Very susceptible plant for me. I was sent pollen, but it didn't work either.
Today I read a post from Laaz that he never found variation among its seedlings. It could be strictly nucellar.
For Laaz it bears fruit within a year after germination, I've never heard that before from anyone in Europe.
I wonder what it could be.... Nevertheless, I will not give up. But I am now focusing more on microcitrus species.

I know this thread in the forum for tropical fruits. I started it :)
I forgot that I have a different nickname here........


Walt

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Re: CRISPR for sale
« Reply #6 on: 2019-09-25, 12:35:25 AM »
Yes, a strange plant.  Several people have posted that seedlings from it have not been precocius for them.  Several others have posted that seedlings from Laaz have been precocius.  Ganerally all posts have been from experienced growers that I respect.
I have a row of seedlings from seeds Laaz sent last fall.  I grew them inside last winter and planted them outside last spring.  They have grown well and are sturdy plants.  I would love to be making crosses with them next year.  I'll report on them whenever they do bloom/

ImGrimmer

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Re: CRISPR for sale
« Reply #7 on: 2019-09-25, 08:55:53 AM »
Back to Synthego.
What is their job? I don`t understand what this site is for. Do they offer to snip in a gene in a given genome?
Maybe a native speaker is able to find out.