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Messages - Dominic J

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Plant Breeding / Re: Rhubarb breeding
« on: 2022-05-29, 01:01:47 PM »
R. palmatum is a diploid.  I believe there are other diploid accessions but RB 50 was the only one I requested.

Rheum palmatum is sometimes diploid, sometimes tetraploid.

Plant Breeding / Re: Rhubarb breeding
« on: 2022-05-21, 11:29:14 AM »
These are the accessions I received from the genebank.  RB 50 is the palmatum accession that I have. Some of these do not use PI #'s.

   USDA Acc.   RB #   Name(s)   Origin/Country      Height cm   
1  PI 666019  - W6 - Rheum x rhabarbarum   PI 666019   RB 33   Minn No. 8   USA   Selections from selfed seedling progenies from "Ruby" donated to D. Dabis, U of Minn.   85   
 4  RB 19  - W6 - Rheum x rhabarbarum   RB 19 / MSU 5   RB 19   New Zealand   New Zealand   Mette donates to Malcolm Tarbottom, Kelburn Holdings Ltd., New Zealand   99.7   
 5  PI 666009  - W6 - Rheum x rhabarbarum   PI 666009      Moore's Red-Right-Thru   Nova Scotia, CA   Selection from Sutton   89.8   
 6  PI 666005  - W6 - Rheum x rhabarbarum   PI 666005   RB 11   OR 23   USA   Bred in Covallis   86   Most genetically different from Cawood Delight
 8  RB 3  - W6 - Rheum x rhabarbarum   RB 3 /MSU 51   RB 03   Linnaeus 137/31   USA   unknown   59 / 76   
 9  RB 50  - W6 - Rheum palmatum   RB 50    RB 50   2/24 UK (R. palmatum)   UK      101   Falls outside the dendogram, outside the condu
 3  PI 666012  - W6 - Rheum x rhabarbarum   PI 666012   RB 20   Caewood Delight   Cawood Selby, North Yorkshire   Cawood Delight   85   Most genetically different from OR 23

RB 19, RB 3, and RB 50 are the three that survived over the winter and are growing this year. 

I have several seedlings from the EFN seed that I will be transplanting outdoors in the next week, and the one cultivar I had previously is in full flower now.  I am letting it go to seed, though the seed would be fully self pollinated.
Are these diploids, though? I looked up RB 50, it didn't seem to mention anything about ploidy. The rest, being rhabarbarum, are presumably all tetraploids.

Cucurbits / Re: Hulless pumpkin
« on: 2022-04-19, 11:02:37 AM »
I picked mine when ripe, waited maybe a week or two before de-seeding it, did not have any such issues. Did fail to dry it properly though and had them mold in their bag, but with a bleach treatment I was able to salvage most of them.

Cucurbits / Hulless pumpkin
« on: 2022-04-05, 05:49:24 PM »
Doesn't seem to be a whole lot of people interested in pumpkin?

I haven't grown pumpkins a lot, not a big fun of pumpkin pie/muffings, but once I tasted 'Kakai' seeds, I was blown away. We'd roast pumpkin seeds pretty much every year, but I always found them pretty unpleasant and bland. But these hulless pumpkins... were something else. Started harvesting seeds last year from my patch that had 'Kakai', 'Lady Gedova', and a few others. The seed yield feels pretty poor, though. Lots of growing space used up for a handful of seeds.

I figure I could try to breed in PM resistance into these, along with better flesh flavor (though honestly, Kakai's flesh didn't seem all that bad to me), but mostly, I'd like to work on these seed yields. I was wondering if anyone had any ideas to help improve that.

Stuart (1983) claims that a single recessive gene conditions the existence of a thin, parchment like seedcoat. So that trait is pretty easy to work with.

Plant Breeding / Re: Rhubarb breeding
« on: 2022-04-03, 07:35:13 PM »
Who knows, without doing something like counting guard cells or having advanced genetic tools.

All the diploid accessions in the USDA genebank are listed as other species.  All the Rheum x rharbarbarum accessions listed are tetraploid. 

I purchased seeds from the Experimental Farm Network this year and so far have 5 seedlings started.

I am waiting for emergence from the USDA accessions planted last year, but am fearing that none survived the winter.

Can you give PI numbers? None of those I looked at gave ploidy info.

R. palmatum sounds very similar to rhubarbarum. I suspect it would cross with rhubarbarum readily.

Plant Breeding / Re: Rhubarb breeding
« on: 2022-04-03, 12:00:20 PM »
Common rhubarb is said to be tetraploid, as with most of the genus, though in some articles there are somewhat contradictory accounts of diploid populations among other related species. I wonder if there are confirmed diploid accessions from the genus. Or otherwise if anther culture could be used to yield diploid common rhubarb.

Given that it is recommended to cut floral stalks to maintain vigor and that this can be labor intensive, I could see benefits to sterile triploid varieties. Assuming the parent plants are all edible and thus that the diploid species aren't toxic.

Plant Breeding / Re: Hardy Chili (Capsicum flexuosum)
« on: 2021-01-09, 07:58:18 AM »
I think it can cross with chinense and annum, few others pretty easily. It also has some self incompatibility issues which could be used for breeding projects.
 Offspring won't be as hardy unless you do a bunch of backcrosses. Still extends season.

I don't think the species itself is that amazingly hardy, but as you said, it could extend the season a little, by helping them resist late or early frost (or near-frost). This alone can potentially add about 20% growing days around here, on a not so atypical year.

Plant Breeding / Re: Hardy Chili (Capsicum flexuosum)
« on: 2021-01-08, 07:20:28 AM »
Intriguing. Does it hybridize with common peppers?

Cucurbits / Re: Citrullus sexual abnomalities
« on: 2020-12-29, 01:27:53 PM »
Tetsukabuto (futsu kurokawa (moschata) x green delicious (maxima))is male sterile!
It is not a CMS but a interspecific crossbreeding very large.
He required a different male for his fecondation!
But her male sterility is normaly recessiv.

Yea, to clarify I'm looking for CMS in commercial varieties for watermelon (or other crops that I breed with). I found one paper that talks about male sterility in tomato, onion, peppers, gourds, and a few others.

Commercial or genebank, really, either works. I'd just love for hybrid breeding to be more easily accessible to smaller scale breeders.

Cucurbits / Re: Citrullus sexual abnomalities
« on: 2020-12-27, 12:38:33 PM »
Tomatoes, onions, potatoes. Not sure about specific varieties, but it makes predictable hybridizing much simpler so it's apparently an industry standard.

Yea, I have a general idea of which species it is reported to have been used in, but I can't find any mention of specific commercial cultivars. All the articles talk about coded breeding lines.

I would like to get the male sterility alleles for my own hybrid breeding projects, but I have no clue where to look.

Plant Breeding / Re: Seed or ovule culture contracting
« on: 2020-12-25, 06:48:37 PM »
Tissue culture is one thing, ovule culture is an extra complexity.

I'm getting set up for tissue culture, but anther and ovule culture I don't think I'll be toying with right away.

Plant Breeding / Re: Walnut species hybridization project
« on: 2020-12-25, 06:33:38 PM »
That's a fair point.

Plant Breeding / Re: Walnut species hybridization project
« on: 2020-12-25, 07:15:01 AM »
Does anyone have any information on triploid walnuts?

I've seen some articles where they want to, and succeed, in creating triploid walnuts. But they don't say *why* they want to do that, or what the results are in terms of fruit bearing. I'm assuming these guys are after the lumber, and not the fruits? Because triploids are generally seedless. For example, one article I read about triploid peaches (or was it cherries?) trying to have pitless fruits just had hollow pitted fruits. But with nut trees, the kernel's what we are after...

In some species, tetraploids have bigger fruits than diploids, in others, they have smaller fruits. Anyone have any insights as far as tetraploid walnuts go?

Cucurbits / Re: Citrullus sexual abnomalities
« on: 2020-12-25, 07:08:26 AM »
I've come across some articles saying that male sterility was used by many breeders to produce hybrids, in watermelons among other crops.

Would anyone know any such hybrid on the market that uses male sterility?

Plant Breeding / Re: Rhubarb breeding
« on: 2020-12-07, 09:58:51 AM »
Rhubarb is not always cooked, though. A lot of people eat them raw, either with sugar or with salt. As for the death incident in 1919, the leaves had been cooked and remained toxic nonetheless.

I'm not sure cooking actually does anything, either to petioles or leaf blades. OA isn't denatured by heat, and the leaves remain toxic despite cooking. It's plausible some molecules might be broken down by cooking, but doesn't look like any of the ones of interest are.

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